Micro-Credential in Plant Cell & Tissue Culture Techniques

Duration

2 Months (8 Weeks)

Mode of Delivery

Online/ Face to Face

Online

Application

Programme Overview

Plant tissue culturing techniques is a lecture, tutorial and practical based subject that have become immensely significant in the agricultural community. Plant tissue culture is effectively used as typical, broad-scale techniques by the biotechnological/ agricultural industry. The course is essential to understand the methods used to grow large numbers of plant cells, in vitro, in an aseptic and closely controlled environment. Therefore, it is necessary to produce large number of plants that are genetically identical to a parent plant, as well as to one another. The course also covers the history of plant tissue culture development, the basic requirements of a plant tissue culture laboratory, preparation of culture medium, the establishment of aseptic tissues, factors affecting the growth of the different types of in vitro cultures, development of disease free plants, protoplast cultures, somatic hybridization, haploid plant cultures and cryopreservation techniques.

Learning Outcomes

On completion of the course participants should be able to:

  • Describe the fundamental principles of Plant Tissue Culture Techniques.
  • Demonstrate understanding of Plant Tissue Culture Techniques in practical activities.
  • Analyze the concept of plant tissue culture in a group.
  • Discuss on the problems encountered in Plant Tissue Culture process and micro-propagation.
  • Present orally on Plant Tissue Culture Techniques

Course Progression

Credits from this Micro-Credential Programme are transferable to the following full academic programme:

  • Degree of Biotechnology

Assessments

  • Coursework 80%
  • Final Examination 20%

Study Plan for Micro-Credential in Plant Cell & Tissue Culture Techniques

Week 1

BASIC PRINCIPLES AND TERMINOLOGY OF PLANT TISSUE CULTURE

– Terminology

– Types of cells

– Types of tissue culture

– Advantages and disadvantages of tissue culture technology

BASIC REQUIREMENTS OF A TISSUE CULTURE LABORATORY

– Laboratory design and development

– Media preparation room

– Culture room

– Sterilization room

– Equipment and apparatus

Lecture

Week 2

 

ASEPTIC TECHNIQUES IN PLANT TISSUE CULTURE

– Sterilization techniques

– Instrument and equipment

– Aseptic techniques

Practical 1

– Aseptic culture techniques for establishment and maintenance of cultures

 

TISSUE CULTURE MEDIA

– Preparation and handling

– Media composition

– Solidifying agents

– Plant growth regulators

– Establishing aseptic cultures

 

Practical 2

– Explant preparation

 

Lecture, practical

Week 3

 

TISSUE CULTURE TECHNIQUES

– Quantitative improvement

– Qualitative improvement

– Micropropagation

– Cell differentiation

– Aseptic preparation of explant

 

Practical 3

– Preparation of stock solutions of MS basal medium and plant growth regulator stocks

 

Assignment 1

Oral presentation 1

 

TISSUE CULTURE TECHNIQUES

– Selection of explant

– Callus culture

– Plant morphogenesis

– Morphology and characteristics of callus

 

Practical 4

– Callus culture technique, Callus Induction

 

Mid-term test

 

 

 

 

Lecture, practical, Assignment, presentation, test

Week 4

 

TISSUE CULTURE TECHNIQUES

– Procedure of callus culture

– Callus multiplication

– Suspension cultures

– Agitation

– Significance of callus culture

 

CELL SUSPENSION CULTURES

– History and evolution

– Initiation and establishment of cell

  suspension cultures

– Plant cell cultures vs. microbial cultures

 

Lecture

Week 5

 

HAPLOID PRODUCTION

– Androgenesis

– Gynogenesis

– Factors affecting haploid androgenesis

– Diploidization of haploids

 

SOMACLONAL, PROTOCLONAL AND GAMETOCLONAL VARIATION

– Origin of somaclonal variation

– Mechanisms leading to genetic variations

– Applications and limitations of somaclonal

  variation

– Gametoclonal variation

 

Lecture

Week 6

 

SOMATIC HYBRIDIZATION AND CYBRIDIZATION

– Steps involved in somatic hybridization

– Protoplast fusion

– Cybrids or cytoplasmic hybrids

– Applications & limitations of somatic hybridization

 

GERMAPLASM CONSERVATION AND CRYOPRESERVATION

– Methods of germplasm preservation

In vitro preservation

– Cryopreservation

– Steps involved in cryopreservation

 

Assignment 2

Oral presentation 2

 

 

 

Lecture, assignment, presentation

Week 7

 

PLANT CELL CULTURE TECHNOLOGY FOR PRODUCTION OF SECONDARY METABOLITES

– Metabolites

– Secondary metabolites

– Production of secondary metabolites

– Strategies of yield improving of secondary metabolites

 

Practical test

 

Lecture, practical

Week 8

 

Final Examination

Conclusion Session

Awarding of Certificates

Presentation by VC or Representative of VC

 

Why Micro-Credential in Lincoln University College (LUC), Malaysia?

Micro-credentials in Lincoln University College (LUC), Malaysia offer a flexible, targeted, and accelerated pathway of acquiring industry-relevant skills that meet the demands of today’s evolving job market. Designed for students, professionals, and individuals seeking to upskill or reskill, credentials offer learning experiences and formally recognized certifications, with enhanced employability, and serving as stepping stones to higher qualifications. LUC’s micro-credential programs are supported by strong academic frameworks and offer clear progression pathways into full-fledged degree programs. After completing this Micro-credential course, professionals can use their updated skills to apply for promotions or switch to a different job role.

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