Micro-Credential in Fermentation Technology & Bioprocess Engineering

Week 1

Introduction to microbial growth kinetics

·         Isolation, preservation and maintenance of industrial microbes

·         Microbial growth kinetics-

–          metabolism and death:

 a)   Growth patterns

b)   Growth phases

c)    Effect of factors:

     i)  oxygen supply

                  ii) heat generation

d)      Growth kinetics (Monod Equation)

e)      Growth in continuous culture

      (ideal chemostat)  

Lecture

Week 2

Microbial Fermentation: Development & Scale up

·         Recombinant protein production in bench-top fermentors

·         Bioprocess concepts in mammalian cell culture technology

·         Fermentor setup and sterilization

·         various sterilization techniques for solid

·         liquid and gases, aeration and agitation, foam, auxillary equipments

·         Solid state fermentation vs. submerged fermentation

·         Control of various parameters

·         Role of computer in fermentor operation

–           

Lecture, practical

Week 3

Bioprocessing

·         Introduction to cell culture and fermentation technology through applied biochemical engineering.

·         Cell Culture/Fermentation (CCF) Introduction

–          Protein expression in bacterial and mammalian cells: basic concepts and methods for improvements

–          Post translational modifications: protein glycosylation

–          Strain development and improvement

–          Biological basis for industrial fermentations and cell cultures

–          Scale-up strategies in fermentation processes

–          Biomass monitor

–          Anaerobic and aerobic fermentation

–          Types of fermenters, solid state fermentation, process economics, fermentation economics

Lecture, practical

Week 4

Bioreactor

·         Power requirements in bioreactors

·         Oxygen transfer and shear in bioreactors

·         Process goals and bioreactor mode of operation

–          Batch

–          Fed-batch

–          Repeated fed batch\

–          Continuous

–          Perfusion

–          Effluent treatment, scale up and scale down.

Lecture, practical

Week 5

Upstream Process

·         Preparation of bioreactor

·         Inoculum development and sterile techniques

·         Preparation of bioreactor instrumentation devices including:

–          Temperature probes

–          pH and dissolved gas

             probes

·         Inoculation

·         Operation, sampling and analysis

·         Medium design and selection

Downstream Processing

·         Includes the recovery of the products in a pure state and the effluent treatment.

·         Broth conditioning and clarification, removal of microbial cells and solid matter

·         Precipitation, filtration, centrifugation, cell disruptions

·         Chemically assisted solvent extraction, product modification to assist recovery

Lecture, practical

Week 6

Bioreactors Design

·         Tissue engineering

·         Waste Treatment

–          Case study on recent review papers (5 papers) on the selected system will be discussed.

Enzyme immobilization & its application

·         Introduction

·         Techniques used for immobilization:

a)      Adsorption

b)      Covalent binding

c)       Affinity immobilization

d)      Entrapment

·         Materials used for fabrication of immobilization supports

·         Applications and scope

Lecture, practical, tutorial

Week 7

Bioprocess simulation and economics

·         The Good Manufacturing Practice Concept – Guidelines, Quality control, Good automated manufacturing practice

–          Biofuels (ethanol), acids (acetic, citric and gluconic), solvents (acetone, glycerol and butanol)

·         Clinical implications of bioprocesses

–          Antibiotics – example penicillin, streptomycin, tetracycline

–          Amino acids- example lysine, glutamic acid.

Lecture

Week 8

Final Examination

Conclusion Session

Awarding of Certificates

Presentation by VC or Representative of VC