Micro-Credential in Instrumentation
Programme Overview
This subject allows students to understand the principle, function, parts and techniques of instruments. The analysis and characterization of biological and organic compounds by various instrumental techniques namely UV-Visible spectroscopy, Infrared (IR) spectroscopy, nuclear magnetic resonance (NMR), mass spectrometry (MS), agarose gel electrophoresis, thin layer chromatography, gas chromatography, and high performance liquid chromatography are also covered in detail. This subject allows students to understand the principle, function, parts and techniques of instruments. The analysis and characterization of biological and organic compounds by various instrumental techniques namely UV-Visible spectroscopy, Infrared (IR) spectroscopy, nuclear magnetic resonance (NMR), mass spectrometry (MS), agarose gel electrophoresis, thin layer chromatography, gas chromatography, and high performance liquid chromatography are also covered in detail.
Learning Outcomes
On completion of the course participants should be able to:
- Describe the basic principle and techniques for instrument.
- Interpret UV-Visible, Infrared, NMR, MS spectra, TLC, GC and HPLC spectra data and make well-supported conclusion.
- Conduct experiments on instrumentation topic.
- Present assigned topic orally.
Course Progression
Credits from this Micro-Credential Programme are transferable to the following full academic programme:
- Degree of Biotechnology
Assessments
- Coursework 80%
- Final Examination 20%
Study Plan for Micro-Credential in Instrumentation
Week 1 | GENERAL BIOPHYSICS METHODS – Definition of biophysics method – Measurement of pH – Measurement of bacteria colony MICROSCOPY – Light microscopy – Principle – Parts – Function – Operation
– Bright & Dark Field microscopy – Principle – Parts – Function – Operation | Lecture, practical |
Week 2
| MICROSCOPY – Fluorescence microscopy – Principle – Parts – Function – Operation – Phase contrast microscopy – Principle – Parts – Function – Operation UV-VISIBLE (UV-VIS) SPECTROSCOPY – Principle – Parts – Function – Operation | Lecture, practical |
Week 3
| UV-VISIBLE (UV-VIS) SPECTROSCOPY – Measuring concentration of organic compound – Measuring concentration of nucleic acids and UV-Visible (UV-Vis) spectroscopy – Interpretation of the UV-Visible spectra
Oral presentation 1
BEER-LAMBERT LAW – Principle – Limitations
– The Beer-Lambert law and its limitation
| Lecture, practical, presentation |
Week 4
| INFRARED (IR) SPECTROSCOPY – Fundamental of infrared spectroscopy – Interpretation of the Infrared (IR) spectra – Structural information – Functional group
– Quantitative determination of a single compound by infrared spectroscopy
NUCLEAR MAGNETIC RESONANCE (NMR) SPECTROSCOPY – Fundamental of nuclear magnetic resonance (NMR) spectroscopy – Key principles of NMR – 2-dimensional NMR – 3-dimensional NMR – Multi-dimensional NMR – Biological macromolecule structural information from NMR Assignment
| Lecture, practical, assignment |
Week 5
| MASS SPECTROMETRY (MS) – Fundamental of mass spectrometry (MS) – Interpretation of the MS spectra – Structural analysis of bio-molecules by MS
CENTRIFUGATION – Basic Principle of centrifugation – Instrumentation of ultracentrifuge (preparative and analytical) – Factors affecting sedimentation velocity
| Lecture, practical |
Week 6
| AGAROSE GEL ELECTROPHORESIS – Properties of agarose gel – Migration of nucleic acids in agarose gel – Procedure for run agarose gel Electrophoresis
THIN LAYER CHROMATOGRAPHY – Principle – Parts – Function – Operation – Analysis – Applications
GAS CHROMATOGRAPHY – Principle – Technique – Analysis
– Quantitative analysis of a multi-component mixture by gas chromatography
| Lecture, practical, presentation |
Week 7
| HIGH PERFORMANCE LIQUID CHROMATOGRAPHY – Principle – Technique – Analysis
– Quantitative analysis by high performance liquid chromatography
Practical test | Lecture, practical |
Week 8
| Final Examination | Conclusion Session Awarding of Certificates Presentation by VC or Representative of VC
|
Why Micro-Credential in Lincoln University College (LUC), Malaysia?
Micro-credentials in Lincoln University College (LUC), Malaysia offer a flexible, targeted, and accelerated pathway of acquiring industry-relevant skills that meet the demands of today’s evolving job market. Designed for students, professionals, and individuals seeking to upskill or reskill, credentials offer learning experiences and formally recognized certifications, with enhanced employability, and serving as stepping stones to higher qualifications. LUC’s micro-credential programs are supported by strong academic frameworks and offer clear progression pathways into full-fledged degree programs. After completing this Micro-credential course, professionals can use their updated skills to apply for promotions or switch to a different job role.