Micro-Credential in Recombinant DNA Technology

Week 1

INTRODUCTION TO GENE CLONING

·         What is gene cloning?

·         Basic steps of gene cloning

·         Importance of gene cloning

·         The applications of gene cloning and DNA analysis in Biotechnology

RESTRICTION AND MODIFICATION ENZYMES

·         Restriction enzymes

·         Types of restriction enzyme

·         Cleavage patterns

·         Classification of restriction endonucleases

Lecture

Tutorial

Week 2

RESTRICTION AND MODIFICATION ENZYMES

·         Modification enzymes

·         Modification of restriction enzymes Gel electrophoresis

·         Banding patterns

Practical 1

·         Preparation of competent cells

VECTORS FOR GENE CLONING

·         Plasmids

·         Plasmid classification

·         Bacteriophages

·         Lambda and M13 bacteriophages

Lecture

Practical

Week 3

PURIFICATION OF DNA FROM LIVING CELLS

·         Preparation of total cell DNA

·         Growing and harvesting a bacterial culture

·         Preparing of a cell extract

Assignment 1

Oral presentation 1

PURIFICATION OF DNA FROM LIVING CELLS

·         Preparation of DNA from a cell extract

·         Concentration of DNA samples

·         Preparation of plasmid DNA

·         Preparation of bacteriophages DNA

Practical 2

·         Extraction of genomic DNA using CTAB method

Mid-term test

Lecture

Practical

Test

Week 4

MANIPULATION OF PURIFIED DNA

·         DNA manipulative enzymes

·         Nucleases

·         Ligases

·         Polymerases

THE POLYMERASE CHAIN REACTION

·         The polymerase chain reaction in outline

·         Designing the oligonucleotide primers

·         Working out the correct temperatures

·         After the PCR: studying PCR products

Practical 3

Restriction fragment length polymorphism (RFLP)

Lecture

Practical

Week 5

GEL ELECTROPHORESIS & PCR PRODUCT CLONING

·         Gel electrophoresis of PCR products

·         Cloning PCR products

Practical 4

·         Gel electrophoresis and data analysis

SEQUENCING GENES AND GENOMES

·         The methodology for DNA sequencing

·         Chain termination DNA sequencing

·         Pyrosequencing

·         Genome sequencing

Lecture

Tutorial

Practical

Week 6

GENE EXPRESSION AND FUNCTION

·         Studying the RNA transcript of a gene

·         Detecting the presence of a transcript

·         Transcript mapping by hybridization

·         Transcript analysis by primer extension

GENE EXPRESSION AND FUNCTION

·         Transcript analysis by PCR

·         Studying the regulation of gene expression

·         Identifying protein binding sites

·         Identifying control sequences

Assignment 2

Oral presentation 2

Lecture

Presentation

Assignment

Week 7

GENE CLONING AND DNA ANALYSIS IN MEDICINE

·         Production of recombinant pharmaceuticals

·         Identification of genes responsible for human diseases

·         Gene therapy

Practical test

Lecture

Test

Week 8

Final Examination

Conclusion Session

Awarding of Certificates

Presentation by VC or Representative of VC